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regions included in a bicistronic vector enhances and stabilizes follistatin gene expressions in both transgeniccells and transgenic mice

Xiaoming HU,Jing GUO,Chunling BAI,Zhuying WEI,Li GAO,Tingmao HU,Shorgan BOU,Guangpeng LI

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 1,   Pages 87-96 doi: 10.15302/J-FASE-2016087

Abstract: Remarkably, protein expression in transgenic cells with the pMAR-FST vector was significantly higherExogenous was expressed in all of the pMAR-FST transgenic mice at F , F and F .Total muscle growth in F mice was significantly greater than in wild-type mice, with larger musclesin fore and hind limbs of transgenic mice. pMAR-FST transgenic mice were also found with more evenlymice.

Keywords： safety of transgenic     bicistron gene transfer body     transgenic mice     muscle development    

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Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 3,   Pages 179-184 doi: 10.15302/J-FASE-2014020

Abstract: One potential method is to produce transgenic livestock that make modified milk containing high concentrationSubsequently, we injected the BAC construct into pronuclei of mouse fertilized embryos and generated transgenicmice.mice and the concentration of rhBCHE in the milk of individual mice ranged from 76±12 to 159±28 mg·LOur results pave the way for making transgenic livestock to produce large quantities of rhBCHE.

Keywords： recombinant human butyrylcholinesterase (rhBCHE)     human lactoferrin bacterial artificial chromosome (hLF BAC)     transgenicmice     milk    

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Construction of a universal recombinant expression vector that regulates the expression of human lysozyme in milk

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

Frontiers of Agricultural Science and Engineering 2018, Volume 5, Issue 3,   Pages 382-389 doi: 10.15302/J-FASE-2018211

Abstract:

The mammary gland provides a novel method for producing recombinant proteins in milk of transgenicgenomic DNA was microinjected into pronuclei of fertilized mouse embryos, to successfully generate two transgenic

Keywords： BAC recombinant methods     gene expression     human lysozyme     transgenic mice     milk expression vector    

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conventional mature B cells and compromised specific antibody response in bovine immunoglobulin μ heavy-chain transgenicmice

Min ZHANG,Xueqian CHENG,Dan CHU,Jingwen LIANG,Yi SUN,Li MA,Beilei XU,Min ZHENG,Meili WANG,Liming REN,Xiaoxiang HU,Qingyong MENG,Ran ZHANG,Ying GUO,Yunping DAI,Robert AITKEN,Ning LI,Yaofeng ZHAO

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 2,   Pages 158-173 doi: 10.15302/J-FASE-2014015

Abstract: mice.Although the serum concentrations of endogenous IgM and IgG in the transgenic mice were significantlyThese transgenic mice also exhibited a lower frequency of unique complementarity determining region 3Compared to the WT mice, the transgenic mice had a significantly higher percentage of mouse IgM-expressingFinally, we showed that the transgenic mice were deficient in a specific antibody response to antigen

Keywords： bovine Ig μ heavy-chain     transgenic mice     B cell development     allelic exclusion     immune response     Ig repertoire    

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Advances of Transgenic Breeding on Rapeseed (Brassica napus)

Guan Chunyun

Strategic Study of CAE 2002, Volume 4, Issue 8,   Pages 34-39

Abstract: It described advances of transgenic breeding of rapeseed (Brassica napus) in Oil Crops Institute, HunanThe. paper dealt with Bt-transgenic insect-resistant rapeseed line, transgenic sterile line, restorerline and their hybrid, transgenic high oleic acid content rapeseed line and transgenic high oil content

Keywords： rapeseed     transgenic     breeding    

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Enlighenments for China from the Industrialization of the Transgenic Atlantic Salmon in the US

Hu Wei and Zhu Zuoyan

Strategic Study of CAE 2016, Volume 18, Issue 3,   Pages 105-109 doi: 10.15302/J-SSCAE-2016.03.018

Abstract: the US Food and Drug Administration (FDA) approved the "all fish" growth hormone (gh) transgenicgenetically engineered livestock approved for human consumption and is considered as breakthrough of transgenicThe US FDA made this landmark decision after 20 years rigorous bio-safety assessment of the transgenicBoth the scientific research behind the creation of this transgenic product and the active participation

Keywords： transgenic     Atlantic salmon     industrialization     enlightenments    

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Construction of multiple shRNA vectors targeting PEDV and TGEV and production of transgenic SCNT porcine

Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 1,   Pages 66-72 doi: 10.15302/J-FASE-2018229

Abstract: transfected into porcine fetal fibroblasts, G418 resistant transfectants were confirmed by PCR and transgenicThese results have paved the way for future production of marker-free transgenic resistant to PEDV and

Keywords： piglet diarrhea     RNAi     PEDV     TGEV     transgenic resistance breeding    

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Carbon dots-based fluorescence sensor for two-photon imaging of pH in diabetic mice

Frontiers of Chemical Science and Engineering 2023, Volume 17, Issue 3,   Pages 298-306 doi: 10.1007/s11705-022-2212-9

Abstract: CA-CDs has been successfully applied for two-photon imaging of the pH in liver and kidney of diabetic miceImaging results showed that the pH value in kidney of diabetic mice was lower than that of the normalmice, while the pH value in liver of diabetic mice was almost the same as that of the normal mice.

Keywords： carbon dots     two-photon imaging     pH     diabetic mice    

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Inhibitory activity of Bifidobacterium adolescent combined with cisplatin on melanoma in mice and its

HUANG Hongying, LIU Guangchao, QI Yijun, DU Yaowu, CHEN Jugao, MA Yuanfang

Frontiers of Medicine 2008, Volume 2, Issue 2,   Pages 186-190 doi: 10.1007/s11684-008-0035-9

Abstract: inhibitory activity of Bifidobacterium adolescent combined with cisplatin on the growth of melanoma (B16) in miceC57 mice were inoculated with B16 cancer cells to construct mouse model of melanoma and treated withThe ratio of inhibitory activity reached 74.45% when the mice were treated by bifidobacterium combinedthe inhibitory activity on B16 cell proliferation is correlated with the enhanced immune activity in mice
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Particulate matter 2.5 triggers airway inflammation and bronchial hyperresponsiveness in mice by activating

Frontiers of Medicine 2021, Volume 15, Issue 5,   Pages 750-766 doi: 10.1007/s11684-021-0839-4

Abstract: acetylation, airway inflammation, and bronchial hyperresponsiveness were deteriorated in SIRT2 knockout mice

Keywords： particulate matter 2.5     sirtuin 2     p65     airway inflammation     bronchial hyperresponsiveness     triptolide    

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Immunogenicity and protective immunity against otitis media caused by pneumococcus in mice of Hib conjugate

Zeyu Chen,Rong Guo,Jianghong Xu,Chuangjun Qiu

Frontiers of Medicine 2016, Volume 10, Issue 4,   Pages 490-498 doi: 10.1007/s11684-016-0470-y

Abstract: polysaccharide conjugate vaccine with the pneumococcal surface adhesin A (PsaA) protein carrier in young miceA total of 15 young mice aged 3 weeks to 5 weeks were immunized with the conjugate vaccine, and another15 young mice of the same age were immunized with the licensed Hib-tetanus toxoid (TT) vaccine.Furthermore, the third group of 15 young mice was inoculated with phosphate buffer saline as control.The immunized mice were inoculated with pneumococcus in the middle ear.

Keywords： conjugate vaccine     pneumococcal surface adhesin A     Hemophilus influenzae b     immunogenicity     otitis media    

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Gene therapy for hemophilia B mice with scAAV8-LP1-hFIX

Wei Lu,Qingzhang Zhou,Hao Yang,Hao Wang,Yexing Gu,Qi Shen,Jinglun Xue,Xiaoyan Dong,Jinzhong Chen

Frontiers of Medicine 2016, Volume 10, Issue 2,   Pages 212-218 doi: 10.1007/s11684-016-0438-y

Abstract:

Hemophilia B is a hemorrhagic disease caused by the deficiency of clotting factor IX (FIX). Gene therapy might be the ultimate strategy for the disease. However, two main problems that should be solved in gene therapy for hemophilia B are immunity and safety. Self-complementary adeno-associated virus serotype 8 (scAAV8), a non-human primate AAV featuring low immunogenicity and high transfection efficiency in liver cells, might be a potential vector for hemophilia B gene therapy. A strong liver-specific promoter-1 (LP1) was inserted and mutant human FIX Arg338Ala was introduced into plasmid scAAV8-LP1 to develop an optimized AAV8 vector that expresses human clotting factor FIX (hFIX). The efficiency of scAAV8-LP1-hFIX administered through normal systemic injection or hydrodynamic injection was compared. A high expression was achieved using hydrodynamic injection, and the peak hFIX expression levels in the 5×1011 and 1×1011 virus genome (vg) cohorts were 31.94% and 25.02% of normal level, respectively, at 60 days post-injection. From the perspective of long-term (200 days) expression, both injection methods presented promising results with the concentration value maintained above 4% of normal plasma. The results were further verified by enzyme-linked immunosorbent assay and activated partial thromboplastin time. Our study provides a potential gene therapy method for hemophilia B.

Keywords： hemophilia B     AAV8     hFIX     gene therapy    

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Overexpression of netrin-1 improves neurological outcomes in mice following transient middle cerebral

Haiyan LU, Yongting WANG, Falei YUAN, Jianrong LIU, Lili ZENG, Guo-Yuan YANG

Frontiers of Medicine 2011, Volume 5, Issue 1,   Pages 86-93 doi: 10.1007/s11684-011-0118-x

Abstract: The level of NT-1 was increased in the AAV-NT-1-transduced tMCAO mice compared with the control mice.Furthermore, the neurobehavioral outcomes were significantly improved in AAV-NT-1-transduced mice compared

Keywords： adeno-associated virus     angiogenesis     gene transfer     ischemia     middle cerebral artery occlusion     netrin-1    

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Long-term correction of hemorrhagic diathesis in hemophilia A mice by an AAV-delivered hybrid FVIII composed

Frontiers of Medicine 2022, Volume 16, Issue 4,   Pages 584-595 doi: 10.1007/s11684-021-0844-7

Abstract: (hLC), and rat light chain (rLC) were transfected into cells and hydrodynamically injected into HA micePurified AAV viruses were intravenously injected into HA mice at two doses.Notably, the HA mice undergoing the AAV-delivered hHC+ rLC treatment exhibited a visibly higher activity+ rLC) via an AAV-delivered gene therapy substantially improved the hemorrhagic diathesis of the HA mice

Keywords： hemophilia A     adeno-associated virus (AAV)     human/rat hybrid factor VIII     gene therapy     dual chain strategy    

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Development of the expressed immunoglobulin μ chain repertoire during maturation of mice B cells

Jingwen LIANG,Yingfeng LUO,Yi SUN,Meng LEI,Bing ZHANG,Songnian HU,Yaofeng ZHAO

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 3,   Pages 201-213 doi: 10.15302/J-FASE-2014017

Abstract: In the bone marrow and spleen, the developing B cell populations undergo both negative and positive selections to shape their B cell receptor repertoire. To gain insight into the shift of the immunoglobulin heavy (IgH) chain repertoire during B cell development, we undertook large scale Ig μ chain repertoire analysis of pre-B, immature B and spleen B cell populations. We found that the majority of V gene segments, V families, J and D gene segments, were observed to have significantly different usage frequencies when three B cell populations were compared, but the usage profile of the V D, and J genes between different B cell populations showed high correlations. In both productive and nonproductive rearrangements, the length of CDRH3 shortened significantly on average when B cells entered the periphery. However, the CDRH3 length distribution of nonproductive rearrangements did not follow a Gaussian distribution, but decreased successively in the order 3 -2, 3 -1 and 3 , suggesting a direct correlation between mRNA stability and CDRH3 length patterns of nonproductive rearrangements. Further analysis of the individual components comprising CDRH3 of productive rearrangements indicated that the decrease in CDRH3 length was largely due to the reduction of N addition at the 5′ and 3′ junctions. Moreover, with development, the amino acid content of CDRH3 progressed toward fewer positively charged and nonpolar residues but more polar residues. All these data indicated that the expressed Ig μ chain repertoire, especially the repertoire of CDRH3, was fine-tuned when B cells passed through several checkpoints of selection during the process of maturation.

Keywords： repertoire     complementarity determining region 3 of the IgH chain (CDRH3)     immunoglobulin     heavy chain     variable region    

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Title Author Date Type Operation

regions included in a bicistronic vector enhances and stabilizes follistatin gene expressions in both transgeniccells and transgenic mice

Xiaoming HU,Jing GUO,Chunling BAI,Zhuying WEI,Li GAO,Tingmao HU,Shorgan BOU,Guangpeng LI

Journal Article

Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

Journal Article

Construction of a universal recombinant expression vector that regulates the expression of human lysozyme in milk

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

Journal Article

conventional mature B cells and compromised specific antibody response in bovine immunoglobulin μ heavy-chain transgenicmice

Min ZHANG,Xueqian CHENG,Dan CHU,Jingwen LIANG,Yi SUN,Li MA,Beilei XU,Min ZHENG,Meili WANG,Liming REN,Xiaoxiang HU,Qingyong MENG,Ran ZHANG,Ying GUO,Yunping DAI,Robert AITKEN,Ning LI,Yaofeng ZHAO

Journal Article

Advances of Transgenic Breeding on Rapeseed (Brassica napus)

Guan Chunyun

Journal Article

Enlighenments for China from the Industrialization of the Transgenic Atlantic Salmon in the US

Hu Wei and Zhu Zuoyan

Journal Article

Construction of multiple shRNA vectors targeting PEDV and TGEV and production of transgenic SCNT porcine

Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG

Journal Article

Carbon dots-based fluorescence sensor for two-photon imaging of pH in diabetic mice

Journal Article

Inhibitory activity of Bifidobacterium adolescent combined with cisplatin on melanoma in mice and its

HUANG Hongying, LIU Guangchao, QI Yijun, DU Yaowu, CHEN Jugao, MA Yuanfang

Journal Article

Particulate matter 2.5 triggers airway inflammation and bronchial hyperresponsiveness in mice by activating

Journal Article

Immunogenicity and protective immunity against otitis media caused by pneumococcus in mice of Hib conjugate

Zeyu Chen,Rong Guo,Jianghong Xu,Chuangjun Qiu

Journal Article

Gene therapy for hemophilia B mice with scAAV8-LP1-hFIX

Wei Lu,Qingzhang Zhou,Hao Yang,Hao Wang,Yexing Gu,Qi Shen,Jinglun Xue,Xiaoyan Dong,Jinzhong Chen

Journal Article

Overexpression of netrin-1 improves neurological outcomes in mice following transient middle cerebral

Haiyan LU, Yongting WANG, Falei YUAN, Jianrong LIU, Lili ZENG, Guo-Yuan YANG

Journal Article

Long-term correction of hemorrhagic diathesis in hemophilia A mice by an AAV-delivered hybrid FVIII composed

Journal Article

Development of the expressed immunoglobulin μ chain repertoire during maturation of mice B cells

Jingwen LIANG,Yingfeng LUO,Yi SUN,Meng LEI,Bing ZHANG,Songnian HU,Yaofeng ZHAO

Journal Article